Journal: Frontiers in Veterinary Science

Article Title: Unrevealing the therapeutic potential of artesunate against emerging zoonotic Babesia microti infection in the murine model

doi: 10.3389/fvets.2024.1383291

Figure Lengend Snippet: Regulating effect of artesunate (AS) after 4 weeks of treatment. (A) Shows protein levels of inflammation markers including SICAM-1, ICAM-1, and C-reactive protein and proapoptotic factor Bax and anti-apoptotic factor Bcl-2. (B) Shows a bar graph of the percentage of band intensity relative protein expression compared within the control group (CG), the B. microti (BM) group, and the experimental groups B. microti + artesunate 2 mg/kg low-dose group (LG), B . microti + artesunate 4 mg/kg medium-dose group, and 8 mg/kg high-dose group. (a) SICAM-1, (b) ICAM-1, (c) CRP, (d) BAX, (e) Bcl-2 bar graph, with asterisks representing a higher level of significance, “*” ( p < 0.05) “**”( p < 0.01) ***”( p < 0.001)”****”( p < 0.0001).

Article Snippet: 3 , ICAM-1 , ICAM-1; 1:2000; Catalogue# HY-P80502; Med Chem Express, China.

Techniques: Expressing, Control

Journal: International journal of cardiology

Article Title: Enhanced external counterpulsation inhibits endothelial apoptosis via modulation of BIRC2 and Apaf-1 genes in porcine hypercholesterolemia.

doi: 10.1016/j.ijcard.2013.11.033

Figure Lengend Snippet: Fig. 2. Isolation and identification of vascular endothelial cells from the aortic endothelium with collagenase. Representative photomicrographs (×400) of isolated cells treated histocytochemically with CD31 antibody and incubated with DiI-Ac-LDL. Cells with cytomembrane and cytoplasmic staining of amber color indicate vascular endothelial cells (black arrow), and cells with red fluorescence were identified as viable vascular endothelial cells (white arrow), which consisted of the majority of total treated cells, indicating a successful en- dothelial cell isolation from aortic endothelium.

Article Snippet: Then mouse polyclonal CD31 antibody (at 1:100 dilution; Boster Biological Technology, Inc, Wuhan, China) was applied and incubated at 37 °C for 1 h, then stained with SABC, enhanced by DAB, dehydrated in a graded ethanol series, and followed by dimethylbenzene treatment to improve transparency, and finally mounted with neutral gum.

Techniques: Isolation, Incubation, Staining, Cell Isolation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways

doi: 10.3389/fcimb.2020.00196

Figure Lengend Snippet: Effects of Histone H3 antibody on LPS-induced NOD2 and VSIG4 signal transduction in vitro . Cells were treated the same way as . (A,B) Immunofluorescence was used to detect the expression of NOD2 in cytoplasm. Relative fluorescent intensity of NOD2 in cytoplasm was presented as mean ± SDs of three independent experiments. (C–E) The protein levels of RIP2, VSIG4, NLRP3, caspase-1, GSDMD, IL-1β, IL-18 in ANA-1 cells. Results were presented as mean ± SDs of three repetitions. * P < 0.05, compared with control group; # P < 0.05, compared with LPS group.

Article Snippet: GAPDH, NOD2, RIP2 antibodies were obtained from Proteintech (Wuhan, China).

Techniques: Transduction, In Vitro, Immunofluorescence, Expressing, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways

doi: 10.3389/fcimb.2020.00196

Figure Lengend Snippet: Effect of histone H3 antibody on NOD2, VSIG4, and pyroptosis in mice. All mice were modeled by LPS (10 mg/kg) except for control group. H3 antibody (20 mg/kg) was given in LPS + H3 antibody group 2 h before sepsis model protocol. All mice were sacrificed after 24 h. (A,B) ELISA results of IL-1β, IL-18 in mice serum. (C–K) Effect of histone H3 antibody on NOD2, RIP2, VSIG4, NLRP3, caspase-1 protein levels in liver, intestine, lung, and kidney. Results were presented as mean ± SDs of three repetitions. * P < 0.05, compared with control group; # P < 0.05, compared with LPS group.

Article Snippet: GAPDH, NOD2, RIP2 antibodies were obtained from Proteintech (Wuhan, China).

Techniques: Control, Enzyme-linked Immunosorbent Assay

Journal: Physiological Reports

Article Title: TRPV4 functional status in cystic cells regulates cystogenesis in autosomal recessive polycystic kidney disease during variations in dietary potassium

doi: 10.14814/phy2.15641

Figure Lengend Snippet: TRPV4 activity regulates subcellular AQP2 distribution in cystic cells of PCK453 rats. (a) Representative confocal images showing AQP2 (pseudocolor red) distribution in kidney sections of PCK453 rats fed regular (0.9%K + ), high KCl (5%K + ), and high KB/C (5%K + , bicarbonate: citrate as 4:1) diets for 1 month. Nuclear Dapi staining is shown with pseudocolor blue. Areas with cystic (1) and non‐dilated collecting duct (2) are shown below at higher magnification. The averaged intensities of AQP2‐reporting fluorescent signals around the apical area in cystic (b) and non‐dilated collecting duct (c) cells from the conditions in (a). For each individual cell the fluorescent signals were normalized to their corresponding maximal value.

Article Snippet: Sections were incubated overnight at +4°C with anti‐AQP2‐ATTO Fluor‐550 (1:200, Alomone Labs; Cat. # AQP2‐002‐AO) and anti‐TRPV4 (1:500, Alomone labs; Cat.#.

Techniques: Activity Assay, Staining

Journal: Physiological Reports

Article Title: TRPV4 functional status in cystic cells regulates cystogenesis in autosomal recessive polycystic kidney disease during variations in dietary potassium

doi: 10.14814/phy2.15641

Figure Lengend Snippet: TRPV4 activity is inversely related to cAMP levels in cystic cells of PCK453 rats. Summary graph comparing dispersion (decrease by 50% from maximum) of AQP2‐reporting signal in non‐dilated collecting duct versus cystic cells in kidney sections of PCK453 rats fed regular (0.9%K + ), high KCl (5%K + ), and high KB/C (5%K + , bicarbonate: citrate as 4:1) diets for 1 month. Bars and whiskers represent SE and SD, respectively. Mean and median values are denoted with lines. Numbers of each experimental groups are shown below. Kidney sections from at least 4 different animals were used for each tested group. *Significant changes ( p < 0.05, one‐way ANOVA with post hoc Tukey test) between groups shown with brackets on the top.

Article Snippet: Sections were incubated overnight at +4°C with anti‐AQP2‐ATTO Fluor‐550 (1:200, Alomone Labs; Cat. # AQP2‐002‐AO) and anti‐TRPV4 (1:500, Alomone labs; Cat.#.

Techniques: Activity Assay, Dispersion